| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1109-1 | ||||
Resumo:Fructooligosaccharides (FOS) are unconventional sugars naturally found in plant foods, but these oligosaccharides can also be synthesized from sucrose by the action of fructosyltransferase (FTase - E.C.2.4.1.9) which hydrolyses sucrose, releasing glucose and fructose, and through a transfer of the free fructose molecule to another sucrose molecule, and the FOS are synthesized. These short-chain oligosaccharides have prebiotic functions and are beneficial to human health by promoting the growth of probiotics. In this context, the present work aimed to analyze the production of FOS from sucrose using the enzyme fructosyltransferase produced by the fungus Aspergillus aculeatus through Fractional Factorial Design. A. aculeatus FTase was obtained from cultivation in Czapek liquid medium supplemented with soybean meal 1.5% (w/v) and incubated for 6 days in BOD at 28ºC, the mycelium was separated by filtration with the aid of a vacuum pump, followed by freezing and grinding. The FTase enzyme was purified from the mycelial extract through a DEAE-Sepharose chromatographic column. The production of FOS was obtained by estimation from the dosages of glucose and reducing sugars carried out in each trial of the experimental design, and expressed in gL⁻¹. Eleven tests were carried out to study the FOS production, in the case of a 2^4-1 fractional factorial design with 3 central points, the 4 variables were: pH (5.0 - 6.0), temperature (45 ºC - 65 ºC), sucrose concentration (1.0 – 2.0 M) and enzymatic reaction time (12 h – 36 h) with FTase, and the statistical analysis of the results was performed using the software Statistica v 7.0. The parameters temperature (45 ºC), sucrose concentration (2 M) and 36 h of reaction time with FTase showed a significant effect on FOS production (p<0.10). The values of the pH parameter studied in the experimental design did not directly influence the production of FOS, only temperature, sucrose and enzymatic reaction time were significant for its production. Thus, this study by Fractional Factorial Design indicated which parameters are relevant to favor the production of FOS from sucrose using the FTase from A. aculeatus isolated from Atlantic Forest biome of Parana. Palavras-chave: fungus, Fractional Factorial Design, fructooligosaccharides, fructosyltransferase Agência de fomento:CAPES, CNPq, Fundação Araucária and UNIOESTE | |||||